Coding

Part:BBa_K5210006:Design

Designed by: yitian zhou   Group: iGEM24_NMU-China   (2024-09-26)


LuxF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 81
    Illegal PstI site found at 76
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 76
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 478
    Illegal NgoMIV site found at 522
    Illegal AgeI site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

In our luminescence circuit,LuxF is controlled by Pompc,which accecpts osmotic regulation.In case of high osmolarity in the medium, the protein kinase EnvZ, located in the cytoplasmic membrane,phosphorylates the transcription factor OmpR. Phosphorylated OmpR binds the ompC promoter and activates the transcription.

References